顯微鏡下的蓋亞

Seeing Gaia under Microscope
顯微鏡下的蓋亞

顯微鏡:嗨,蓋亞!你近況如何? 蓋亞:我感覺很棒!看,那些細胞移動時所留下的軌跡,讓其他細胞有「跡」可循,很快地自組織成奇幻的排列模式。

James Lovelock在1972年提出的蓋亞假說,認為「生命與生態系間的自我調節反饋機制,使地球得以維持在一個適合居住的狀態。」在細胞生態系統中,細胞的移動軌跡作為對其鄰居的反饋機制,改變了細胞與細胞間,亦或是細胞與環境間的互動方式。

材料與方法: 在控溫37°C、5% 二氧化碳和濕度90 - 100%的環境中,透過Zeiss Axio Observer 7倒置顯微鏡搭配Zeiss 20x/0.8 M27複消色差透鏡,以15分鐘為間隔進行縮時攝影,觀察在細胞膜上帶有螢光蛋白標定的活細胞。

Microscope: Hey Gaia! How are you doing? Gaia: I am feeling wonderful! See, those migratory tracks are leaving information for cells to self-organize into magical patterns soon.

The Gaia hypothesis by James Lovelock (1972) postulates that “the Earth is maintained in a habitable state by self-regulating feedback mechanisms involving organisms tightly coupled to their environment.” In the cellular ecosystem, cells leave behind migratory tracks as a feedback mechanism to their neighbors. Cells gather this feedback from other cells to change their space exploration.

Materials and Methods: Cells expressing fluorescent membrane marker were cultured and imaged under Zeiss Axio Observer 7 inverted microscope under controlled temperature (37 °C), atmosphere (5% CO2), and humidity (90 - 100%). Images were taken every 15 minutes using a Zeiss 20x/0.8 M27 Plan-Apochromat objective.